RAN Antibody (N-term)

SKU: orb36887

Description

Purified rabbit polyclonal antibody.

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics,Developmental Biology,Infectious Diseases,Primary Antibodies;Cell Biology;Signal Transduction;Viral process;Host-virus interaction

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution Range: WB: 1:1000, IHC-P: 1:50-100, FACS: 1:10-50
• Reactivity: Human, Mouse
• Predicted Reactivity: C. elegans, Other, Rat, Zebrafish

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB20229
• Immunogen: Synthetic Peptide
• Target: RAN
• Molecular Weight: 24423
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-GTP-binding nuclear protein Ran antibody, anti-GTPase Ran antibody, anti-Ras-related nuclear protein antibody, anti-Ras-like protein TC4 antibody, anti-Androgen receptor-associated protein 24 antibody, anti-OK/SW-cl.81 antibody, anti-ARA24 antibody, anti-RAN antibody

Western blot analysis of RAN Antibody (N-term) in HL-60 cell line lysates (35 ug/lane). RAN (arrow) was detected using the purified Pab.

Western blot analysis of RAN antibody (N-term) in NIH-3T3 cell line lysates (35 ug/lane). RAN (arrow) was detected using the purified Pab.

Western blot analysis of RAN (arrow) using rabbit polyclonal RAN Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RAN gene.

RAN Antibody (N-term) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human brain tissue reacted with RAN Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Quick Database Links

Gene Symbol

RAN

UniProt

P62826

RefSeq (Protein):NP_006316.1