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Recombinant Aurora B (Proliferation Marker) Antibody

SKU: orb2319848

Description

Recombinant Aurora B (Proliferation Marker) Antibody

Research Area

,Signal Transduction|Transcription Factors

Images & Validation

Tested ApplicationsIHC
Dilution RangeImmunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.
ReactivityHuman
Application Notes
Positive Control: HeLa cells. Tonsil, Placenta, Hodgkin's Lymphoma or Colon Carcinoma.

Key Properties

Antibody TypeRecombinant Antibody
HostRabbit
ClonalityMonoclonal
IsotypeIgG
Clone No.AURKB/3121R
ImmunogenRecombinant fragment (around aa 89-251) of human Aurora B protein (exact sequence is proprietary)
TargetAURKB
Source/Expression SystemHEK293 cells
Molecular Weight39kDa
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
ConcentrationPurified Ab WITHOUT BSA and Azide at 1.0mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

AIK2; AIM-1; ARK-2; AurB; AURKB; Aurora-1; Aurora and Ipl1 like midbody associated protein 1; Aurora kinase B; Aurora-B; Aurora-related kinase 2; Aurora/IPL1-related kinase 2; IPL1; Protein phosphatase 1 regulatory subunit 48 (PPP1R48); Serine/threonine-protein kinase 12; Serine/threonine-protein kinase aurora-B; STK1; STK12; STK5

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

Protocol Information

IHC
Immunohistochemistry
View Protocol

Available Sizes

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100 μg
$ 660.00
Free Secondary Antibody (20 ul)0/0

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DispatchUsually dispatched within 5-10 working days
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