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Recombinant Aurora B (Proliferation Marker) Antibody

SKU: orb607855

Description

Mouse monoclonal antibody to Aurora B (Proliferation Marker)

Research Area

Product Categories/Products/Antibodies,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics,Product Categories/Antibodies,Signal Transduction|Transcription Factors

Images & Validation

Tested ApplicationsIHC
Dilution RangeImmunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.
ReactivityHuman
Application Notes
Positive Control: HeLa cells. Tonsil, Placenta, Hodgkin s Lymphoma or Colon Carcinoma.

Key Properties

Antibody TypeRecombinant Antibody
HostMouse
ClonalityMonoclonal
IsotypeIgG1
Clone No.rAURKB/1592
ImmunogenRecombinant fragment (around aa 89-251) of human Aurora B protein (exact sequence is proprietary)
TargetAURKB
Source/Expression SystemHEK293 cells
Molecular Weight39kDa
ConjugationUnconjugated

Storage & Handling

StorageAntibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Form/Appearance200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
Buffer/Preservatives200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
ConcentrationPurified Ab with BSA and Azide at 200ug/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

AIK2; AIM-1; ARK-2; AurB; AURKB; Aurora-1; Aurora and Ipl1 like midbody associated protein 1; Aurora kinase B; Aurora-B; Aurora-related kinase 2; Aurora/IPL1-related kinase 2; IPL1; Protein phosphatase 1 regulatory subunit 48 (PPP1R48); Serine/threonine-protein kinase 12; Serine/threonine-protein kinase aurora-B; STK1; STK12; STK5

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

Protocol Information

IHC
Immunohistochemistry
View Protocol

Available Sizes

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100 μg
$ 660.00
Free Secondary Antibody (20 ul)0/0

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DispatchUsually dispatched within 2 to 3 weeks.
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