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Description
Research Area
,Basal Cell Marker|BBB VCAM-1 Signaling|Breast Cancer|Cardiovascular|Colon Cancer|Developmental Biology|Immunology|Infectious Disease|Signal Transduction|Transcription Factors
Images & Validation
−
| Tested Applications | ELISA, FC, IHC |
|---|---|
| Dilution Range | Flow Cytometry (0.5-1ug/million cells); ELISA (For coating, order antibody without BSA); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined. |
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Recombinant Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | rCTNNB1/1507 |
| Immunogen | Recombinant human beta-Catenin (p120) protein fragment |
| Target | CTNNB1 |
| Source/Expression System | HEK293 cells |
| Molecular Weight | 92kDa |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml. |
| Concentration | Purified Ab with BSA and Azide at 200ug/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Cadherin associated protein, beta 1 88kDa, Catenin beta-1, CATNB, CHBCAT, CTNNB1
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Protocol Information
IHC
Immunohistochemistry
FC
Flow Cytometry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)





