Recombinant CD137 / 4-1BB / TNFRSF9 Antibody

SKU: orb2315027

Description

Recombinant CD137 / 4-1BB / TNFRSF9 Antibody

Research Area

,AKT Signaling|B Cell Markers|Cardiovascular|Cytokine Signaling|Immunology

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, WB
• Dilution Range: ELISA (For coating, order antibody without BSA);,Immunofluorescence (1-2ug/ml); Flow Cytometry (1-2ug/million cells); Western Blot (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Reactivity: Human
• Application Notes:
  Positive Control: HEK293 cells, Jurkat whole cell lysate or recombinant human CD137 protein (WB). Human kidney cancer, liver, stomach or thyroid cancer tissue (IHC-P). Expressed on the surface of activated T-cells.

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: 4-1BB/4552R
• Immunogen: Recombinant fragment (around aa 19-188) of human CD137 protein (exact sequence is proprietary)
• Target: TNFRSF9
• Source/Expression System: CHO cells
• Molecular Weight: 32kDa (monomer); 85kDa (dimer)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Concentration: Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

4-1BB Ligand Receptor T Cell; Antigen 4-1BB Homolog; CDw137; HLDA VI; Homolog of mouse 4 1BB; induced by lymphocyte activation (ILA); Interleukin activated receptor homolog of mouse Ly63; T-cell antigen 4-1BB / ILA homolog; Tumor necrosis factor receptor superfamily member 9

Quick Database Links

Gene Symbol

TNFRSF9

UniProt

Q07011

NCBI Gene ID

3604