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Recombinant CD137 / 4-1BB / TNFRSF9 Antibody

SKU: orb751313

Description

Rabbit monoclonal antibody to TNFRSF9

Research Area

Product Categories/Products/Antibodies,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics,Product Categories/Antibodies,AKT Signaling|B Cell Markers|Cardiovascular|Cytokine Signaling|Immunology

Images & Validation

Tested ApplicationsELISA, FC, IF, IHC, WB
Dilution RangeELISA (For coating, order antibody without BSA);,Immunofluorescence (1-2ug/ml); Flow Cytometry (1-2ug/million cells); Western Blot (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
ReactivityHuman
Application Notes
Positive Control: HEK293 cells, Jurkat whole cell lysate or recombinant human CD137 protein (WB). Human kidney cancer, liver, stomach or thyroid cancer tissue (IHC-P). Expressed on the surface of activated T-cells.

Key Properties

Antibody TypeRecombinant Antibody
HostRabbit
ClonalityMonoclonal
IsotypeIgG
Clone No.4-1BB/4552R
ImmunogenRecombinant fragment (around aa 19-188) of human CD137 protein (exact sequence is proprietary)
TargetTNFRSF9
Source/Expression SystemCHO cells
Molecular Weight32kDa (monomer); 85kDa (dimer)
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance200ug/ml of Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
Buffer/Preservatives200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
Concentration200 μg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Anti-4-1BB Ligand Receptor T Cell antibody, anti-Antigen 4-1BB Homolog antibody, anti-CDw137 antibody, anti-HLDA VI antibody, anti-Homolog of mouse 4 1BB antibody, anti-induced by lymphocyte activation (ILA) antibody, anti-Interleukin activated receptor homolog of mouse Ly63 antibody, anti-T-cell antigen 4-1BB / ILA homolog antibody, anti-Tumor necrosis factor receptor superfamily member 9 antibody

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
FC
Flow Cytometry
View Protocol
IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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100 μg
$ 660.00
Free Secondary Antibody (20 ul)0/0

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DispatchUsually dispatched within 5-10 working days
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