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Recombinant CD31 / PECAM-1 (Endothelial Cell Marker) Antibody
Description
Research Area
Images & Validation
−
| Tested Applications | IHC |
|---|---|
| Dilution Range | Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined. |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Recombinant Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | rPECAM1/8832 |
| Immunogen | Recombinant fragment (around aa 625-738) of human CD31 protein (exact sequence is proprietary) |
| Target | PECAM-1 |
| Source/Expression System | HEK293 cells |
| Molecular Weight | ~100kDa (endothelium) and ~130kDa (platelets)� |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml. |
| Concentration | Purified Ab WITHOUT BSA and Azide at 1.0mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Recombinant CD31 / PECAM-1 (Endothelial Cell Marker) Antibody (orb2311683)
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