Recombinant CD31 / PECAM-1 (Endothelial Cell Marker) Antibody

SKU: orb2315908

Description

Recombinant CD31 / PECAM-1 (Endothelial Cell Marker) Antibody

Research Area

,Angiogenesis|Cardiovascular|Endothelial Cell Marker|Immunology

Images & Validation

• Tested Applications: FC, IHC, WB
• Dilution Range: Western Blot (1-2ug/ml); Flow Cytometry (1-2ug/million cells);Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
• Reactivity: Human, Rat
• Application Notes:
  Positive Control: THP-1, Jurkat cells. Tonsil or Angiosarcoma.

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: C31/2876R
• Immunogen: Human full-length Recombinant CD31 protein
• Target: PECAM-1
• Source/Expression System: HEK293 cells
• Molecular Weight: ~100kDa (endothelium) and ~130kDa (platelets)'
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Concentration: Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

EndoCAM; PECA1; Platelet Endothelial Cell Adhesion Molecule 1; GPIIA'

Quick Database Links

Gene Symbol

PECAM-1

UniProt

P16284

NCBI Gene ID

5175