Recombinant CD3e (T-Cell Marker) Antibody

SKU: orb607841

Description

Mouse monoclonal antibody to CD3e (T-Cell Marker)

Research Area

Product Categories/Products/Antibodies/Primary Antibodies,Immunology,Product Categories/Antibodies,Product Categories/Products/Antibodies,Epigenetics,Cardiovascular|Hematopoietic Stem Cells|Immunology|PD-1 blockade immunotherapy

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA); Western Blot (1-2ug/ml); ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Reactivity: Human
• Application Notes:
  Positive Control: Jurkat cells. Human tonsil or lymph node

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: rC3e/1931
• Immunogen: A recombinant human CD3e protein fragment (around aa 23-119) (exact sequence is proprietary)
• Target: CD3E
• Source/Expression System: HEK293 cells
• Molecular Weight: 20kDa
• Conjugation: Unconjugated

Storage & Handling

• Storage: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
• Form/Appearance: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Buffer/Preservatives: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Concentration: Purified Ab with BSA and Azide at 200ug/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD3 epsilon; CD3 TCR complex; T cell antigen receptor complex epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; T3E; TCRE; TiT3 complex

Quick Database Links

Gene Symbol

CD3E

UniProt

P07766

NCBI Gene ID

916