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Description
Research Area
,Autophagy|Developmental Biology
Images & Validation
−
| Tested Applications | FC, IF, IHC, WB |
|---|---|
| Dilution Range | Flow Cytometry (1-2ug/million cells);Western Blot (1-2ug/ml);Immunofluorescence (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined. |
| Reactivity | Bovine, Canine, Equine, Feline, Ferret, Goat, Hamster, Human, Monkey, Mouse, Porcine, Rat, Sheep |
| Application Notes |
Key Properties
−| Antibody Type | Recombinant Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone No. | KRT18/2819R |
| Immunogen | Recombinant human full-length KRT18 protein |
| Target | KRT18 |
| Source/Expression System | HEK293 cells |
| Molecular Weight | 45kDa |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml. |
| Concentration | Purified Ab WITHOUT BSA and Azide at 1.0mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Cell Proliferation-inducing Gene 46 Protein; CK18; CYK18Cytokeratin Endo B; K18; Keratin-18; Kerd; KRT18
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
IF
Immunofluorescence












