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Recombinant Cytokeratin 8/18 Antibody

SKU: orb751340

Description

Rabbit monoclonal antibody to KRT8 & KRT18

Research Area

Product Categories/Products/Antibodies/Primary Antibodies,Infectious Diseases,Product Categories/Antibodies,Product Categories/Products/Antibodies,Epigenetics

Images & Validation

Tested ApplicationsIHC
Dilution RangeImmunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.
ReactivityHuman
Application Notes
Positive Control: MCF-7 or A431 cells. Skin, Colon, lung or breast carcinoma.

Key Properties

Antibody TypeRecombinant Antibody
HostRabbit
ClonalityMonoclonal
IsotypeIgG
Clone No.KRT8.18/2297R
ImmunogenRecombinant full-length human KRT8/18 protein
TargetKRT8
Source/Expression SystemHEK293 cells
Molecular Weight52.5kDa (CK8); 45kDa (CK18)
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
Buffer/Preservatives200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
Concentration200 μg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Anti-CARD2 antibody, anti-CK8 antibody, anti-CYK8 antibody, anti-CYKER antibody, anti-Cytokeratin Endo A antibody, anti-DreK8 antibody, anti-EndoA antibody, anti-K2C8 antibody, anti-K8 antibody, anti-Keratin 8 antibody, anti-Krt 2.8 antibody, anti-KRT8 antibody, anti-Type-II Keratin Kb8. Cell Proliferation-inducing Gene 46 Protein antibody, anti-CK18 antibody, anti-CYK18Cytokeratin Endo B antibody, anti-K18 antibody, anti-Keratin-18 antibody, anti-Kerd antibody, anti-KRT18 antibody

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

IHC
Immunohistochemistry
View Protocol

Recombinant Cytokeratin 8/18 Antibody (orb751340)

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Available Sizes

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100 μg
$ 660.00
DispatchUsually dispatched within 5-10 working days
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