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Description
Images & Validation
−Key Properties
−| Source | Escherichia coli |
|---|---|
| Expression System | Escherichia coli |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by inducing IL-10 secretion of human COLO 205 cells is less than 0.3 ng/ml, corresponding to a specific activity of > 3.3 x106 IU/mg. |
| Endotoxins | Less than 1 EU/ug of rHuIL-22 as determined by LAL method. |
| MW | Approximately 33.6 kDa, non-disulfide-linked homodimeric protein containing of two 147 amino acid polypeptide chains. |
| Purity | > 97 % by SDS-PAGE and HPLC analyses. |
| Protein Sequence | MAPISSHCRL DKSNFQQPYI TNRTFMLAKE ASLADNNTDV RLIGEKLFHG VSMSERCYLM KQVLNFTLEE VLFPQSDRFQ PYMQEVVPFL ARLSNRLSTC HIEGDDLHIQ RNVQKLKDTV KKLGESGEIK AIGELDLLFM SLRNACI |
Storage & Handling
−| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 5.0. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Unconjugated
> 97 % by SDS-PAGE and HPLC analyses
16.6 kDa
10 μg, 100 μg, 500 μg

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UniProt Details
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Protocol Information
Recombinant Human Interleukin-22 (orb1906326)
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