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Description
Images & Validation
−Key Properties
−| Source | Escherichia coli |
|---|---|
| Expression System | Escherichia coli |
| Biological Activity | Fully biologically active when compared to standard. rHuIL-7 stimulates proliferation of PHA-activated human peripheral blood mononuclear cell (PBMC). The specific activity of Recombinant Human IL-7 is > 1.0 x108 Units/mg, which is calibrated against human IL-7 WHO Standard (NIBSC code: 90/530). |
| Endotoxins | Less than 1 EU/ug of rHuIL-7 as determined by LAL method. |
| MW | Approximately 17.4 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids. |
| Purity | > 97% by SDS-PAGE and HPLC analyses. |
Storage & Handling
−| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, 150 mM NaCl, pH 7.4. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Protocol Information
Recombinant Human Interleukin-7 (orb1906335)
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