Recombinant Human Transforming Growth Factor - beta 1

SKU: orb1906258

Description

Recombinant Human Transforming Growth Factor - beta 1

Key Properties

• Source: Chinese Hamster Ovary cell line, CHO
• Expression System: Chinese Hamster Ovary cell line, CHO
• Biological Activity: Measured by its ability to inhibit the IL-4-dependent proliferation of HT-2 mouse T cells. The ED50 for this effect is 0.04-0.2 ng/ml. The specific activity of rHuTGF-β1 is approximately 2.5 x104 U/μg, which is calibrated against human TGF-β1 Standard.
• Endotoxins: Less than 0.1 EU/ug of rHuTGF-β1 as determined by LAL method.
• MW: Apparent molecular mass of 24 kDa in SDS-PAGE under non-reducing conditions, 12 kDa under reducing conditions, a disulfide-linked homodimer of two 112 amino acid glycosylated polypeptide chains.
• Purity: > 97 % by SDS-PAGE analyses.
• Protein Sequence: Ala279-Ser390; Accession # P01137

Storage & Handling

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Form/Appearance: Lyophilized from 0.2 μm filtered concentrated solution in 35 % Acetonitrile and 0.1 % TFA.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only