Recombinant mouse anti vimentin

SKU: orb669776

Description

Mouse monoclonal antibody to Vimentin

Research Area

Cancer Research, Cardiovascular Research, Cytoskeleton, Immunology, Neuroscience, Stem Cell Research

Images & Validation

• Tested Applications: ICC, IHC, WB
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: RV204
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Biorbyt accepts no liability for any inaccuracies or omissions in this information.
• Disclaimer: For research use only

Indirect immunofluorescence staining of normal human dermal fibroblasts in tissue culture with orb669776 (diluted 1:250), showing the specific cytoskeletal pattern of vimentin intermediate filaments.

Indirect immunofluorescence staining of human kidney tissue section with orb669776 (diluted 1:1000), showing the specific pattern of vimentin in the mesenchymal cell types, such as fibroblasts in the connective tissue, podocytes, and endothelial cells in blood vessels. As expected, no reactivity is seen in the epithelial cell compartment

Indirect immunofluorescence staining of human kidney tissue section with orb669776 (diluted 1:1000), showing the specific pattern of vimentin in the mesenchymal cell types, such as fibroblasts in the connective tissue, podocytes, and endothelial cells in blood vessels. As expected, no reactivity is seen in the epithelial cell compartment

Immunostaining of human paraffin embedded tissue section of placenta with orb669776 (diluted 1:100), showing the specific pattern of vimentin in the mesenchymal cell types, such as fibroblasts in the connective tissue, and endothelial cells in blood vessels. As expected, no reactivity is seen in the epithelial cell compartment

Immunostaining of human paraffin embedded tissue section of testis with orb669776 (diluted 1:100), showing the specific pattern of vimentin in the mesenchymal cell types, such as fibroblasts in the connective tissue, endothelial cells in blood vessels and Sertoli cells.

Immunostaining of human paraffin embedded tissue sections of human colon with orb669776 (diluted 1:100), showing the specific pattern of vimentin in the mesenchymal cell types, such as fibroblasts in the connective tissue, and endothelial cells in blood vessels. As expected, no reactivity is seen in the epithelial cell compartment.

Immunostaining of human paraffin embedded tissue sections of human kidney with orb669776 (diluted 1:100), showing the specific pattern of vimentin in the mesenchymal cell types, such as fibroblasts in the connective tissue, and podocytes. As expected, no reactivity is seen in the epithelial cell compartment.

Immunostaining of human paraffin embedded tissue sections of human small intestine with orb669776 (diluted 1:100), showing the specific pattern of vimentin in the mesenchymal cell types, such as fibroblasts in the connective tissue. As expected, no reactivity is seen in the epithelial cell compartment.

Immunostaining of human paraffin embedded tissue sections of human spleen with orb669776 (diluted 1:100), showing the specific pattern of vimentin in the mesenchymal cell types.

Non-reduced and reduced SDS-PAGE of orb669776 showing its purity.

Quick Database Links

UniProt

P08670