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Description
Images & Validation
−Key Properties
−| Source | Escherichia coli |
|---|---|
| Expression System | Escherichia coli |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of the proliferation of IL-6-dependent murine 7TD1 cells is less than 0.02 ng/ml, corresponding to a specific activity of > 5 x107 IU/mg. |
| Endotoxins | Less than 1 EU/ug of rMuIL-6 as determined by LAL method. |
| MW | Approximately 21.7 kDa, a single non-glycosylated polypeptide chain containing 188 amino acids. |
| Purity | > 97 % by SDS-PAGE and HPLC analyses. |
| Protein Sequence | MFPTSQVRRG DFTEDTTPNR PVYTTSQVGG LITHVLWEIV EMRKELCNGN SDCMNNDDAL AENNLKLPEI QRNDGCYQTG YNQEICLLKI SSGLLEYHSY LEYMKNNLKD NKKDKARVLQ RDTETLIHIF NQEVKDLHKI VLPTPISNAL LTDKLESQKE WLRTKTIQFI LKSLEEFLKV TLRSTRQT |
Storage & Handling
−| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution in 30 mM Acetic Acid, pH 3.0, 150 mM NaCl, 5 % Trehalose, 0.02 % Tween-20. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Murine Oncostatin M protein [orb107791]
HPLC, SDS-PAGE
Unconjugated
> 96 % by SDS-PAGE and HPLC analyses
20.3 kDa
10 μg, 100 μg, 500 μgRecombinant Murine Interleukin-6 (rMuIL-6) [orb1495008]
> 97% by SDS-PAGE and HPLC analyses.
Approximately 21.7 kDa, a single non-glycosylated polypeptide chain containing 187 amino acids
Escherichia coli.
1 mg, 10 μg, 2 μgRat Oncostatin M protein [orb107792]
HPLC, SDS-PAGE
Unconjugated
> 96 % by SDS-PAGE and HPLC analyses
24.3 kDa
10 μg, 100 μg, 500 μgRecombinant Human OSM(his Tag) [orb1921820]
≥95%
26.8 kDa
CHO Cell
1 mg, 500 μg, 50 μg, 100 μg, 200 μg

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Protocol Information
Recombinant Murine Interleukin-6 (orb1906206)
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