Recombinant Topoisomerase II alpha (Proliferation & Drug-Resistance Marker) Antibody

SKU: orb751323

Description

Rabbit monoclonal antibody to TOP2A

Research Area

Product Categories/Products/Antibodies,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics,Product Categories/Antibodies,Nuclear Marker

Images & Validation

• Tested Applications: IHC
• Dilution Range: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Reactivity: Human
• Application Notes:
  Positive Control: Human tonsil or esophagus

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: TOP2A/4397R
• Immunogen: Synthetic peptide corresponding to Topo II α residues within aa1431-1531 of Topo II α
• Target: TOP2A
• Source/Expression System: HEK293 cells
• Molecular Weight: 170kDa
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Buffer/Preservatives: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Concentration: 200 μg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-ATP hydrolyzing DNA topoisomerase II alfa antibody, anti-DNA gyrase antibody, anti-DNA topoisomerase (ATP hydrolyzing) antibody, anti-DNA topoisomerase 2 alpha antibody, anti-DNA topoisomerase II 170kD antibody, anti-DNA topoisomerase II alpha antibody, anti-DNA Topoisomerase2 antibody, anti-TOP2A antibody, anti-Topoisomerase DNA II alpha 170kDa antibody, anti-TP2A antibody

Quick Database Links

Gene Symbol

TOP2A

UniProt

P11388

NCBI Gene ID

7153