SOD2 Mouse Monoclonal Antibody

SKU: orb500056

Description

SOD2 Mouse Monoclonal Antibody

Research Area

Antioxidants, Metabolism, Apoptosis, Cell Cycle, Cancer Research, Cardiovascular Research, Hypoxia, Metabolism, Mitochondrial Markers, Cell Biology, Mitochondrial, Cell Biology, Neurodegenerative Disease, Neuroscience

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:200-1000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:20-200
• Reactivity: Human, Mouse
• Predicted Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1κ
• Clone No.: 6C11
• Immunogen: Recombinant human SOD protein
• Target: SOD2
• Molecular Weight: 22 kDa
• Purification: affinity purified by Protein G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

IPO B; Manganese SOD; Superoxide Dismutase 2; Manganese superoxide dismutase; Mn SOD; MNSOD; SOD 2; SOD-2; SOD2; Superoxide dismutase [Mn] mitochondrial; Superoxide dismutase [Mn] mitochondrial precursor; Superoxide dismutase 2 mitochondrial; SODM_HUMAN.

Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (SOD2) Monoclonal Antibody, Unconjugated (orb500056) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (SOD2) Monoclonal Antibody, Unconjugated (orb500056) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human pancreatic cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (SOD2) Monoclonal Antibody, Unconjugated (orb500056) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Sample: Lane 1: Hela (Human) Cell Lysate at 30 ug, Lane 2: 293T (Human) Cell Lysate at 30 ug, Lane 3: Raw264.7 (Mouse) Cell Lysate at 30 ug, Primary: Anti-SOD2 (orb500056) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 22 kD, Observed band size: 22 kD.

Sample: Siha (Human) Cell Lysate at 30 ug, HepG2 (Human) Cell Lysate at 30 ug, K562 (Human) Cell Lysate at 30 ug, U251 (Mouse) Cell Lysate at 30 ug, Primary: Anti-SOD2 (orb500056) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 22 kD, Observed band size: 22 kD.

Quick Database Links

Gene Symbol

SOD2

UniProt

P04179