TLR2 Rabbit Polyclonal Antibody

SKU: orb11487

Description

TLR2 Rabbit Polyclonal Antibody

Research Area

Defensins, Immune System, Inflammation, Inflammatory Mediators, Macrophage, Signal Transduction, TLR Signaling Pathway

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, IF=1:50-200
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Gallus, Mouse, Porcine, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human TLR2 (701-784/784aa)
• Target: TLR2
• Molecular Weight: 89 kDa
• Purification: affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Toll-like receptor 2; Toll like receptor 2; Toll like receptor 2 precursor; Toll-like receptor 2; TLR 2; TLR-2; Toll/interleukin-1 receptor-like protein 4; CD282 antigen; CD282; TIL 4; TIL4; TLR2_HUMAN; Toll/interleukin 1 receptor like 4; Toll/interleukin 1 receptor like protein 4; Toll/interleukin receptor like protein 4.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (TLR2) Polyclonal Antibody, Unconjugated (orb11487) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Spleen (Rat) Lysate at 40 ug, Lane 2: Bronchus (Rat) Lysate at 40 ug, Lane 3: Lung (Rat) Lysate at 40 ug, Primary: Anti-TLR2 (orb11487) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 100 kD, Observed band size: 98 kD.

Quick Database Links

Gene Symbol

TLR2

UniProt

O60603

Ezz, Mohamed Aboul et al. TLR2/4 signaling pathway mediates sperm-induced inflammation in bovine endometrial epithelial cells in vitro PLoS ONE, 14, e0214516 (2019)

PubMed: 30995239

Applications

IHC

Reactivity

Bovine

Bishayi, B. et al. Possible role of Toll-like receptor-2 in the intracellular survival of Staphylococcus aureus in murine peritoneal macrophages: involvement of cytokines and anti-oxidant enzymes Scand J Immunol, 80, 127-143 (2014)

PubMed: 24846691

Applications

WB

Reactivity

Mouse

Kowsar, Rasoul et al. Regulation of innate immune function in bovine oviduct epithelial cells in culture: the homeostatic role of epithelial cells in balancing Th1/Th2 response J Reprod Dev, 59, 470-478 (2013)

PubMed: 23800958

Applications

IHC

Reactivity

Bovine

Rituparna Ghosh 1, Biswadev Bishayi Neutralization of TLR2 in combination with either TNF-α or IL-1β antibody reduces the severity of septic arthritis through STAT3/mTOR signalling in lymphocytes Cell Immunol ., (2024)

PubMed: 39312873

I Akthar, Y Kim, T Umehara, C Kanno Activation of sperm Toll-like receptor 2 induces hyperactivation to enhance the penetration to mucus and uterine glands: a trigger for the uterine inflammatory cascade Frontiers in Immunology, (2024)

PubMed:

Applications

IF

Rituparna Ghosh 1, Biswadev Bishayi Endogenous blocking of TLR2 along with TNF-α and IL-1β ameliorates the severity of the S. aureus arthritis via modulating STAT3/SOCS3 expressions in tissue resident macrophages Microb Pathog, (2024)

PubMed: 38160988

Malinda Hulugalla 1, Alireza Mansouri 1, Elham Waehama 1 2, Ihshan Akthar 1, Akio Miyamoto Semen extender triggers a mild physiological inflammatory response in the uterus without disrupting sperm-uterine immune crosstalk in vitro in cattle J Reprod Dev, (2024)

PubMed: 39647914

Dongxue Ma 1, Ihshan Akthar 1, Taiyo Hashimoto 1, Mohamed A Marey 1 2, Kazuya Kusama 3, Kazuhiko Imakawa 4, Masayuki Shimada 5, Akio Miyamoto Toll-like receptor 2 activation of early divided bovine embryo promotes its viability and development competence in vitro Sci Rep, (2025)

PubMed: 40634450

Applications

IF, WB

Reactivity

Bovine

Effects of Bdellovibrio bacteriovorus HD100 on experimental periodontitis in rats Mol Oral Microbiol, 38, 158-170 (2023)

PubMed: 36495122

Dey, Rajen et al. Ascorbic acid along with ciprofloxacin regulates S. aureus induced microglial inflammatory responses and oxidative stress through TLR-2 and glucocorticoid receptor modulation Inflammopharmacology, 30, 1303-1322 (2022)

PubMed: 35704229