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Description
Research Area
,Apoptosis,Cancer,Chemokines & Cytokines,Growth Factors,Immunology,Innate Immunity,Obesity,Signal Transduction
Images & Validation
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| Application Notes |
|---|
Key Properties
−| Source | E. coli |
|---|---|
| Biological Activity | Induces cell death at a concentration range of 0.01-10ng/ml (ED50= 0.05ng/ml or 2 x 10E7 Units/mg; WEHI 164 cells). |
| Target | TNF |
| Tag | DDDDK Tag |
| Purity | > 95% (SDS-PAGE). Endotoxin level is less than 0.01EU/ μg purified protein (LAL test; Lonza). |
| Protein Sequence | A DDDDK-tag is fused at the N-terminus of human TNF-alpha (aa 85-223). |
Storage & Handling
−| Storage | Stable for at least 1 year after receipt when stored at -20˚°C. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Contains PBS. Reconstitute with 100 μl sterile water. PBS containing at least 0.1% rAlbumin should be used for further dilutions. |
| Concentration | 10 ug size: 0.1 mg/ml after reconstitution.50 ug size: 1 mg/ml after reconstitution. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Tumor Necrosis Factor Ligand Superfamily Member 2, Cachectin
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
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