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Description
Research Area
,Apoptosis,Cancer,Growth Factors,Immunology
Images & Validation
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| Application Notes |
|---|
Key Properties
−| Source | E. coli |
|---|---|
| Biological Activity | Induces apoptosis of human Jurkat T cells in a concentration range of 5-50ng/ml in the presence of 1ug/ml TNF Ligands Enhancer (Prod. No. AG-35B-0001). In the absence of TNF Ligands Enhancer, TRAIL, Soluble (human) (recombinant) is poorly active. |
| Target | TNFSF10 |
| Tag | DDDDK Tag |
| Purity | > 95% (SDS-PAGE). Endotoxin level is less than 0.01EU/ μg purified protein (LAL test; Lonza). |
| Protein Sequence | The extracellular domain of human TRAIL (aa 95-281) is fused at the N-terminus to a DDDDK-tag. |
Storage & Handling
−| Storage | Stable for at least 6 months after receipt when stored at -20˚°C. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Contains PBS. Reconstitute with 100 μl sterile water. |
| Concentration | 0.1mg/ml after reconstitution. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Apo-2L, TNFSF10, CD253
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
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