UBCH10 Antibody

SKU: orb1246818

Description

UBCH10 Antibody

Research Area

,Cancer

Images & Validation

• Tested Applications: ELISA, FC, IF, IP, WB
• Reactivity: Human
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx.19KDa band observed in lysates of cell lines HeLa and HEK293 (calculated MW of 19.7kDa according to NP_008950.1). Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS and MCF7 cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml. Immunoprecipitation: 20KDa band precipitated from mitotic HeLa whole cell lysates using protein-G dynabeads.

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Key Properties

• Antibody Type: Primary Antibody
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-QETYSKQVTSQEP
• Target: UBE2C
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

UBE2C, UBCH10, dJ447F3.2, ubiquitin-conjugating enzyme E2C, ubiquitin-protein ligase C, ubiquitin carrier protein E2-C, cyclin-selective ubiquitin carrier protein, mitotic-specific ubiquitin-conjugating enzyme, RP3-447F3.1

Quick Database Links

Gene Symbol

UBE2C

UniProt

O00762

RefSeq (Protein):NP_001268670.1, NP_861515.1, NP_861517.1, NP_861518.1, NP_008950.1, NP_861516.1