VEGFR2 Rabbit Polyclonal Antibody

SKU: orb221947

Description

VEGFR2 Rabbit Polyclonal Antibody

Research Area

Angiogenesis, Cancer Research, Cancer Research, Cell Markers, Cardiovascular Research, Endothelial Cells, Vascular, Growth Factors, Cancer Research, Host-Virus Interaction, Virology, Hypoxia, Receptor Tyrosne Kinases, Cell Biology, Surface Molecules, Molecular Biology, VEGF Receptors, Vascular, cardiovascular Research

Images & Validation

• Tested Applications: FC
• Dilution Range: Flow-Cyt=1μg/Test
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Porcine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide derived from human VEGFR2 (1001-1100/1356aa)
• Target: KDR
• Molecular Weight: 147 kDa
• Purification: affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Vascular endothelial growth factor receptor 2; KDR; VEGF Receptor 2; CD309; CD309 antigen; Fetal liver kinase 1; FLK-1; FLK1; Kinase insert domain receptor(a type III receptor tyrosine kinase); Kinase insert domain receptor; KRD1; Ly73; Protein tyrosine kinase receptor FLK1; Protein-tyrosine kinase receptor flk-1; Tyrosine kinase growth factor receptor; VEGFR 2; VEGFR; VEGFR-2; VGFR2_HUMAN.

Tissue/Cell: Human esophageal cancer tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-VEGFR2 Polyclonal Antibody, Unconjugated (orb221947) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (orb868589) used at 1:200 dilution for 40 minutes at 37°C. DAPI (5 ug/ml, blue) was used to stain the cell nuclei.

Tissue/Cell: Rat liver, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-VEGFR2/FLK1 Polyclonal, Unconjugated (orb221947) 1:100, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

KDR

UniProt

P35968