Vimentin Antibody

SKU: orb1151558

Description

Rabbit monoclonal antibody to Vimentin

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Epigenetics,Infectious Diseases

Images & Validation

• Tested Applications: IHC-P
• Dilution Range: Immunohistochemistry (FFPE): 1-2ug/ml
• Reactivity: Bovine, Canine, Equine, Feline, Human, Mouse, Rat
• Application Notes:
  Optimal dilution of the recombinant Vimentin antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: Rabbit IgG
• Clone No.: VIM/6430R
• Immunogen: A portion of amino acids 2-466 from the human protein was used as the immunogen for the recombinant Vimentin antibody.
• Purity: Protein A/G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Aliquot the recombinant Vimentin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This recombinant Vimentin antibody is available for research use only.
• Disclaimer: For research use only

IHC staining of FFPE human tonsil tissue with recombinant Vimentin antibody (clone VIM/6430R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human tonsil tissue with recombinant Vimentin antibody (clone VIM/6430R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human tonsil tissue with recombinant Vimentin antibody (clone VIM/6430R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE dog kidney tissue with recombinant Vimentin antibody (clone VIM/6430R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE cat kidney tissue with recombinant Vimentin antibody (clone VIM/6430R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE horse small intestine tissue with recombinant Vimentin antibody (clone VIM/6430R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE horse lung tissue with recombinant Vimentin antibody (clone VIM/6430R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE cow brain tissue with recombinant Vimentin antibody (clone VIM/6430R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human U-87 MG cell lysate with recombinant Vimentin antibody. Predicted molecular weight ~53 kDa.

SDS-PAGE analysis of purified, BSA-free recombinant Vimentin antibody (clone VIM/6430R) as confirmation of integrity and purity.

Quick Database Links

UniProt

P08670