ZAP70 Mouse Monoclonal Antibody

SKU: orb783009

Description

ZAP70 Mouse Monoclonal Antibody

Research Area

CD Markers, Cell Biology, SARS /COVID-19, Tumor Biology, Cancer Research, Tyrosine Kinases, Cell Biology

Images & Validation

• Tested Applications: FC, ICC, WB
• Dilution Range: WB=1:500-2000, ICC/IF=1:25, Flow-Cyt=1:25
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone No.: V4R3
• Immunogen: Recombinant human ZAP70.
• Target: ZAP70
• Molecular Weight: 68 kDa
• Purification: affinity purified by Protein G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ZAP-70; ZAP 70; zeta-associated protein 70; ZAP-70=protein tyrosine kinase Syk homolog {SH2-like and C-terminal kinase domains}; Tyrosine-protein kinase ZAP-70; 70 kDa zeta-associated protein; Syk-related tyrosine kinase. SRK; STD; TZK; ZAP70_HUMAN; Tyrosine-protein kinase ZAP-70; 70 kDa zeta-chain associated protein.

Sample: Lane 1: Jurkat cell lysates, Lane 2: Molt-4 cell lysates, Primary: Anti-ZAP70 (orb783009) at 1/4000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 68 kD, Observed band size: 68 kD.

Blank control: Jurkat. Primary Antibody (green line): Mouse Anti-ZAP70 antibody (orb783009), dilution: 1:25, Isotype Control Antibody (blue line): Mouse IgG, Secondary Antibody: Goat anti-mouse IgG-AF488, dilution: 1:400, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

HeLa cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (ZAP70) monoclonal Antibody, Unconjugated (orb783009) 1:25, 90 minutes at 37°C, followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, Dylight554 Phalloidin (red) was used to stain the cell Cytoplasmic actin. The nuclear counter stain is DAPI (blue).

Quick Database Links

Gene Symbol

ZAP70

UniProt

P43403