Alpha Synuclein Pre-formed Fibrils (Type 1)

SKU: orb1822365

Description

Human Recombinant Alpha Synuclein Pre-formed Fibrils (Type 1)

Research Area

,Neuroscience | Neurodegeneration | Alzheimer's Disease | Tangles & Tau | Neuroscience | Neurodegeneration | Parkinson's Disease | Synuclein | Neuroscience | Neurodegeneration | Multiple System Atrophy

Images & Validation

• Tested Applications: In vitro, In vivo, SDS-PAGE, WB
• Application Notes:
  Certified > 95% pure using SDS-PAGE analysis. Low endotoxin < 5 EU/mL @ 2mg/mL.

Key Properties

• Source: Recombinant
• Expression System: E. coli
• Biological Activity: Endogenous alpha-synuclein phosphorylation. 100 µM alpha synuclein protein monomer (SPR-321) seeded with 10 uM alpha synuclein protein PFF (SPR-322) in 25 µM Thioflavin T (PBS pH 7.4, 100 µl reaction volume) generated a fluorescence intensity of 13,000 Relative Fluorescence Units after incubation at 37°C with shaking at 600 rpm. Fluorescence was measured by excitation at 450 nm and emission at 485 nm on a Molecular Devices Gemini XPS microplate reader.
• Target: Alpha Synuclein
• Reactivity: Human
• Tag: No tag
• Protein Length: Full Length
• MW: ~14.46 kDa
• Purity: > 95%
• Protein Sequence: MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVATVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGSIAA ATGFVKKDQL GKNEEGAPQE GILEDMPVDP DNEAYEMPSE EGYQDYEPEA

Storage & Handling

• Storage: -80°C
• Buffer/Preservatives: PBS
• Concentration: 2 mg/ml or 5 mg/ml
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Alpha synuclein PFFs, Alpha synuclein aggregates, Alpha synuclein PFF, Alpha synuclein protein aggregates, Alpha synuclein aggregates, Alpha-synuclein protein, Non-A beta component of AD amyloid protein, Non-A4 component of amyloid precursor protein, NACP protein, SNCA protein, NACP protein, PARK1 protein, SYN protein, Parkinson's disease familial 1 Protein, Alpha synuclein protein seed

Quick Database Links

Gene Symbol

Alpha Synuclein

UniProt

P37840