AKT1 Monoclonal Antibody

SKU: orb546987

Description

Anti-AKT1 Monoclonal Antibody. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Images & Validation

• Tested Applications: FC, ICC, IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB 1:500-1:2000IHC 1:50-1:200ICC/IF 1:50-1:200IP 1:50FC 1:50. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone No.: EBG-1
• Immunogen: A synthesized peptide derived from human AKT1 Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473.
• Molecular Weight: 56 kDa
• Purification: Affinity-chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Concentration: Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Signal transducer and activator of transcription 3; Acute-phase response factor; STAT3; APRF

Quick Database Links

UniProt

P31749