Anti-Vimentin Purified

SKU: orb44572

Description

Mouse monoclonal antibody to Vimentin

Research Area

Epigenetics, Infectious Diseases, Neuroscience

Images & Validation

• Tested Applications: ICC, IHC-P, IP, WB
• Reactivity: Gallus, Human, Mouse, Porcine, Rat
• Application Notes:
  Immunocytochemistry: Staining technique: (a) fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C; (b) fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C. Positive control: 3T3 murine Swiss albino fibroblast cell line, RBL rat basophilic leukemia cell line. Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/ml, positive tissue: skin fibroblast.Western blotting: Recommended dilution: 1-2 μg/ml.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgM
• Clone No.: VI-10
• Target: Vimentin
• Purity: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
• Purification: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti CTRCT30 antibody, anti Epididymis luminal protein 113 antibody, anti HEL113 antibody, anti VIM antibody, anti Vimentin antibody

Immunoprecipitation of vimentin from HeLa cell lysate by antibody VI-10 and its detection by antibody VI-01. IgM heavy chain (76-92 kDa) and IgM light chain (25-30 kDa) indicated. Mr of vimentin is 57 kDa. Lr = lysate (reducing conditions); Lnr = lysate (non-reducing conditions); IPr = immunoprecipitate (reducing conditions); IPnr = immunoprecipitate (non-reducing conditions).

Immunocytochemistry staining of chicken postnatal erythrocytes. Tubulin (red) was stained with anti-alpha-tubulin (TU-01; cat. no. orb44529), vimentin (green) with anti-Vimentin (VI-10). Nuclei are stained with DAPI (blue).

Immunohistochemistry staining of human skin (paraffin sections) using anti-vimentin (VI-10).

Western blotting analysis of human vimentin using mouse monoclonal antibody VI-10 on lysates of MOLT-4 cell line (low expression), U87 cell line (positive) and Raji cell line (negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-vimentin monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for vimentin at approximately 55 kDa.

Quick Database Links

Gene Symbol

Vimentin

UniProt

P08670

NCBI Gene ID

7431