Anti-Vimentin Purified

SKU: orb44574

Description

Mouse monoclonal antibody to Vimentin

Research Area

Epigenetics, Infectious Diseases, Neuroscience

Images & Validation

• Tested Applications: ELISA, FC, ICC, IHC-P, WB
• Reactivity: Human
• Application Notes:
  ELISA: The antibody VI-RE/1 recognizes different epitope on human vimentin than the antibody VI-01 (IgM). Flow cytometry: Recommended dilution: 1-4 μg/ml. Intracellular staining.Western blotting: Recommended dilution: 1-2 μg/ml.Immunocytochemistry: Recommended dilution: 5-10 μg/ml.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: VI-RE/1
• Immunogen: Bacterially expressed full-length human vimentin
• Target: Vimentin
• Purity: Purified by protein-A affinity chromatography.
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti CTRCT30 antibody, anti Epididymis luminal protein 113 antibody, anti HEL113 antibody, anti VIM antibody, anti Vimentin antibody

Flow cytometry analysis (intracellular staining) of vimentin expression in ESS-1 cells using anti-human vimentin (VI-RE/1) purified, GAM-FITC. Negative control: human lymphocytes.

Immunohistochemistry staining (paraffin sections) of vimentin in human liver using mouse monoclonal antibody VI-RE/1 (orb44574, diluted 1:400), detected with GAM IgG-Alexa Fluor®488 (diluted 1:200; green), cell nuclei stained with PI (1 µg/ml; orange).

Western blotting analysis of human vimentin using mouse monoclonal antibody VI-RE/1 on lysates of MOLT-4 cell line (low expression), U87 cell line (positive) and Raji cell line (negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-vimentin monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for vimentin at approximately 55 kDa.

Quick Database Links

Gene Symbol

Vimentin

UniProt

P08670

NCBI Gene ID

7431