CD11B Antibody

SKU: orb1259324

Description

CD11B Antibody

Research Area

,Cell Cycle,Immunology,Neuroscience,Signal Transduction,Stem Cell

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-330 of human CD11B (NP_001139280.1).
• Target: ITGAM
• Molecular Weight: Observed: 180kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Integrin alpha-M, CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor, CD11b, ITGAM, CD11B, CR3A

Western blot analysis of extracts of various cell lines, using CD11B antibody (orb1259324) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunofluorescence analysis of rat bone marrow using CD11B antibody (orb1259324) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of RAW264.7 cells using CD11B antibody (orb1259324) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of THP-1 cells using CD11B antibody (orb1259324) at dilution of 1:100. Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

ITGAM

UniProt

P11215