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Description
Research Area
Immunology & Inflammation
Images & Validation
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Item 1 of 2
| Application Notes |
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Key Properties
−| Expression System | HEK293 |
|---|---|
| Biological Origin | Human |
| Biological Activity | Humira (Adalimumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human TNF-alpha Protein, His Tag with an affinity constant of 0.244 nM as determined in a SPR assay (Biacore T200) (Routinely tested). |
| Target | TNF-alpha |
| Reactivity | Human |
| Tag | C-6×His |
| Expression Region | Val 77 - Leu 233 |
| Endotoxins | 1.0 EU per μg |
| Conjugation | Unconjugated |
| MW | 18.3 kDa |
| Purity | 95% |
| Protein Sequence | NP_000585.2 |
Storage & Handling
−| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
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| Form/Appearance | Powder |
| Buffer/Preservatives | PBS, pH 7.4 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
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Human TNF alpha His tag protein [orb80108]
FA, HPLC, SDS-PAGE
Unconjugated
> 95.0% as determined by RP-HPLC and analysis by SDS-PAGE
10 μg, 100 μg, 500 μg 
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Quick Database Links
Gene Symbol
TNF-alpha
RefSeq (Protein):NP_000585.2
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_000585.2 |
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
Available Sizes
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