ICAM1 Rabbit Polyclonal Antibody

SKU: orb101051

Description

ICAM1 Rabbit Polyclonal Antibody

Research Area

Product Categories/Products/Antibodies,Endothelial Cell Markers,Cell adhesion molecules,Regulatory T Cells,Adhesion,SARS Coronavirus,B Lymphocytic Lineage,T Lymphocytic Lineage

Images & Validation

• Tested Applications: FC
• Dilution Range: Flow-Cyt=1μg/Test
• Reactivity: Human
• Predicted Reactivity: Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide derived from human CD54/ICAM-1 (451-505/532aa)
• Target: ICAM1
• Molecular Weight: 56 kDa
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BB2; CD54 antigen; CD54; ICAM1; ICAM 1; ICAM-1; Cell surface glycoprotein P3.58; Human rhinovirus receptor; Intercellular adhesion molecule 1; Major group rhinovirus receptor; MALA2; MyD10; P3.58; Surface antigen of activated B cells; ICAM1_HUMAN; MALA-2; MyD10; intercellular adhesion molecule 1 precursor; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; major group rhinovirus receptor.

Blank control (blue): U937 (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-ICAM1 antibody (orb101051), Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

Sample: Lane 1: A431 (Human) Cell Lysate at 30 ug, Lane 2: Huvec (Human) Cell Lysate at 30 ug, Lane 3: HepG2 (Human) Cell Lysate at 30 ug, Lane 4: Siha (Human) Cell Lysate at 30 ug, Lane 5: U937 (Human) Cell Lysate at 30 ug, Primary: Anti-ICAM1 (orb101051) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 58/100 kD, Observed band size: 58 kD.

Sample: Lung (Mouse) Lysate at 40 ug, Spleen (Mouse) Lysate at 40 ug, Lymph node (Mouse) Lysate at 40 ug, Heart (Mouse) Lysate at 40 ug, A549 (Human) Cell Lysate at 40 ug, A431 (Human) Cell Lysate at 40 ug, U937 (Human) Cell Lysate at 40 ug, K562 (Human) Cell Lysate at 40 ug, Primary: Anti-ICAM1 (orb101051) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 56 kD, Observed band size: 56 kD.

Quick Database Links

Gene Symbol

ICAM1

UniProt

P05362