ICAM1 Rabbit Polyclonal Antibody

SKU: orb500976

Description

ICAM1 Rabbit Polyclonal Antibody

Research Area

Product Categories/Products/Antibodies,Endothelial Cell Markers,Cell adhesion molecules,Regulatory T Cells,Adhesion,SARS Coronavirus,B Lymphocytic Lineage,T Lymphocytic Lineage

Images & Validation

• Tested Applications: FC, WB
• Dilution Range: WB=1:500-2000, Flow-Cyt=1μg/Test
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Guinea pig, Porcine, Rabbit, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide derived from human ICAM-1 (180-250/532aa)
• Target: ICAM1
• Molecular Weight: 56 kDa
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BB2; CD54 antigen; CD54; ICAM1; ICAM 1; ICAM-1; Cell surface glycoprotein P3.58; Human rhinovirus receptor; Intercellular adhesion molecule 1; Major group rhinovirus receptor; MALA2; MyD10; P3.58; Surface antigen of activated B cells; ICAM1_HUMAN; MALA-2; MyD10; intercellular adhesion molecule 1 precursor; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; major group rhinovirus receptor.

Blank control (blue): U937 (fixed with 2% paraformaldehyde (10 min)) Primary Antibody: Rabbit Anti-ICAM1 antibody (orb500976), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA.

Sample: A549 Cell (Human) Lysate at 40 ug, Primary: Anti-ICAM1 (orb500976) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 56 kD, Observed band size: 56 kD.

Sample: Raw264.7 cell (Mouse) Lysate at 40 ug, Raji Cell (Human) Lysate at 40 ug, Heart (Mouse) Lysate at 40 ug, Thymus (Mouse) Lysate at 40 ug, Primary: Anti-ICAM1 (orb500976) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 56 kD, Observed band size: 56 kD.

Quick Database Links

Gene Symbol

ICAM1

UniProt

P05362