ICAM1 Rabbit Polyclonal Antibody

SKU: orb101633

Description

ICAM1 Rabbit Polyclonal Antibody

Research Area

Product Categories/Products/Antibodies,Endothelial Cell Markers,Cell adhesion molecules,Regulatory T Cells,Adhesion,SARS Coronavirus,B Lymphocytic Lineage,T Lymphocytic Lineage

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide derived from human CD54/ICAM-1 (51-100/532aa)
• Target: ICAM1
• Molecular Weight: 56 kDa
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BB2; CD54 antigen; CD54; ICAM1; ICAM 1; ICAM-1; Cell surface glycoprotein P3.58; Human rhinovirus receptor; Intercellular adhesion molecule 1; Major group rhinovirus receptor; MALA2; MyD10; P3.58; Surface antigen of activated B cells; ICAM1_HUMAN; MALA-2; MyD10; intercellular adhesion molecule 1 precursor; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; major group rhinovirus receptor..

Blank control (blue line): U937 (fixed with 2% paraformaldehyde for 10 min at room temperature). Primary Antibody (green line): Rabbit Anti-ICAM1 antibody (orb101633), Dilution: 0.2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1 µg/test.

Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ICAM1) Polyclonal Antibody, Unconjugated (orb101633) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: A549 Cell (Human) Lysate at 40 ug, Primary: Anti-ICAM1 (orb101633) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 56 kD, Observed band size: 56 kD.

Sample: Raji Cell (Human) Lysate at 40 ug, Primary: Anti-ICAM1 (orb101633) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 56 kD, Observed band size: 56 kD.

Sample: U937 Cell (Human) Lysate at 40 ug, Primary: Anti-ICAM1 (orb101633) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 56 kD, Observed band size: 60 kD.

Quick Database Links

Gene Symbol

ICAM1

UniProt

P05362