Recombinant S100A4 / Metastasin / Calvasculin (Marker of Tumor Metastasis) Antibody

SKU: orb2316010

This product can be custom made

Description

Research Area

,Dendritic Cell Marker

Images & Validation

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Tested Applications	FC, IF, IHC, WB
Dilution Range	Flow Cytometry (1-2ug/million cells); Western Blot (1-2ug/ml); Immunofluorescence (1-2ug/ml);,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.
Reactivity	Human, Mouse
Application Notes	Positive Control: HeLa, A549 or A375 cells (IF/FACS). HeLa, A549, T98G or A375 cell lysate (WB). Human placenta tissue (IHC).

Key Properties

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Antibody Type	Recombinant Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone No.	rS100A4/1481
Immunogen	Recombinant fragment (around aa 1-200) of human S100A4 protein (exact sequence is proprietary)
Target	S100A4
Source/Expression System	HEK293 cells
Molecular Weight	10-12kDa
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
Concentration	Purified Ab with BSA and Azide at 200ug/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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S100 calcium-binding protein A4; CAPL; Fibroblast specific protein 1 (FSP1); Leukemia multidrug resistance associated protein; Malignant transformation suppression 1 (MTS1); Metastasin; Placental calcium-binding protein

Quality Guarantee

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Quick Database Links

Gene Symbol

S100A4

UniProt

P26447

NCBI Gene ID

6275

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

Documents Download

Datasheet

Product Information

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Protocol Information

Western Blot (IB, immunoblot)

View Protocol

IHC

Immunohistochemistry

Flow Cytometry

Immunofluorescence