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Recombinant S100A4 / Metastasin / Calvasculin (Marker of Tumor Metastasis) Antibody
SKU: orb2316011
Description
Research Area
,Dendritic Cell Marker
Images & Validation
−
| Tested Applications | FC, IF, IHC, WB |
|---|---|
| Dilution Range | Western Blot (1-2ug/ml); Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined. |
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Recombinant Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone No. | S100A4/2750R |
| Immunogen | Recombinant fragment of human S100A4 protein (around aa1-200) (exact sequence is proprietary) |
| Target | S100A4 |
| Source/Expression System | HEK293 cells |
| Molecular Weight | 10-12kDa |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml. |
| Concentration | Purified Ab WITHOUT BSA and Azide at 1.0mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−S100A4; S100 calcium-binding protein A4; Calvasculin; CAPL; Fibroblast specific protein 1 (FSP1); Leukemia multidrug resistance associated protein; Malignant transformation suppression 1 (MTS1); Metastasin; Placental calcium-binding protein
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
IF
Immunofluorescence