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Recombinant S100A4 / Metastasin / Calvasculin (Marker of Tumor Metastasis) Antibody

SKU: orb607692

Description

Mouse monoclonal antibody to S100A4 / Metastasin / Calvasculin (Marker of Tumor Metastasis)

Research Area

Product Categories/Products/Antibodies,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics,Product Categories/Antibodies,Dendritic Cell Marker

Images & Validation

Tested ApplicationsFC, IF, IHC, WB
Dilution RangeFlow Cytometry (1-2ug/million cells); Western Blot (1-2ug/ml); Immunofluorescence (1-2ug/ml);,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.
ReactivityHuman, Mouse
Application Notes
Positive Control: HeLa, A549 or A375 cells (IF/FACS). HeLa, A549, T98G or A375 cell lysate (WB). Human placenta tissue (IHC).

Key Properties

Antibody TypeRecombinant Antibody
HostMouse
ClonalityMonoclonal
IsotypeIgG1
Clone No.rS100A4/1481
ImmunogenRecombinant fragment (around aa 1-200) of human S100A4 protein (exact sequence is proprietary)
TargetS100A4
Source/Expression SystemHEK293 cells
Molecular Weight10-12kDa
ConjugationUnconjugated

Storage & Handling

StorageAntibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Form/Appearance200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
Buffer/Preservatives200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
ConcentrationPurified Ab with BSA and Azide at 200ug/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

S100 calcium-binding protein A4; CAPL; Fibroblast specific protein 1 (FSP1); Leukemia multidrug resistance associated protein; Malignant transformation suppression 1 (MTS1); Metastasin; Placental calcium-binding protein

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
FC
Flow Cytometry
View Protocol
IF
Immunofluorescence
View Protocol

Available Sizes

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100 μg
$ 660.00
Free Secondary Antibody (20 ul)0/0

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DispatchUsually dispatched within 2 to 3 weeks.
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